How To Sterilize lab equipment. Vials – Rubber Stoppers – Autoclave – Distilled water – Isopropyl Alcohol

How To Sterilize lab equipment.

Vials – Rubber Stoppers – Autoclave – Distilled water – Isopropyl Alcohol

Author: Musclechemistry Site Representative thx thx is online now

 

Since a lot of people have asked me about how to sterilize lab equipment, vials etc

I’ve finally decided to write summary on how to do it.
There are a lot of misconceptions about sterilizing lab equipment. I’m going to try to keep it short and simple
but at the same time give you a little deeper understanding.

You should ALWAYS do your own research and not believe things you read on forums, ALWAYS!

Here is a list of the most important things that you’ll need:

How To Sterilize Lab Equiptment

  • Autoclave/pressure cooker
  • Distilled water
  • Isopropyl alcohol (isopropanol) or ethanol (both high concentration)
  • Surgical gloves
  • Surgical mask
    Less important things:
  • Autoclave pouches/bags
  • Autoclave tape
  • Lab wash bottle

    First thing you need is the actual device that you’re going to sterilize your lab equipment with.

    Autoclave = Expensive and can be rather big. You can get a used one cheap, but you can run in to problems with it. This is the most convenient device (except for buying pre-sterilized things)

    Steam sterilizer (autoclave) = That’s essentially a pressure cooker with a gauge, usually 18 liter. This is your best option. You can get one from ebay fairly cheap.

    Pressure cooker = You can find a regular pressure cooker anywhere. Only problem is not all pressure cookers get up to the right pressure/temperature.

    The autoclave/pressure cooker has to reach this temperature/pressure:
    121°C (250°F) 15psi 15min
    or
    132°C (270°F) 30psi 3min

    It’s the steam/pressure/temperature combination that kills off all the “bad things”. So you’re not allowed to cover your beakers/Erlenmeyer/vials with aluminum foil.

    You can also get autoclave bags. Those bags usually have an indicator that changes color if the right temperature is reached. There is also autoclave tape that does the same thing.

    Assuming all the lab equipment/vials (which they are if they are new) are washed and cleaned here is what you do:

    1.Alcohol (preferably high percentage isopropyl alcohol but ethanol works as well) to clean your working area and lab equipment with.

    2.You’ll need distilled water (get a big jug) since you can fill “lab wash bottle” and rinse your lab equipment/glassware after cleaning it with alcohol (so no residue is left, that’s why high grade alcohol is preferred)
    The distilled water is used for the autoclave/pressure cooker as well. It’s used because it won’t leave any minerals/residue after it evaporates which regular water does.

    3.If you decide to get a regular pressure cooker don’t forget to buy a “vegetable steamer basket” since you can’t place your vials/equipment on the bottom of the pressure cooker.
    You don’t need that much water in the pressure cooker. 2.5 – 4cm (1 – 1.5 inches) is usually enough. There should be a little space between the water and the bottom of the “vegetable basket” (You’ll know how much water you’ll need)

    4.Follow the instructions of the pressure cooker or steam sterilizer (autoclave) and just set the time yourself (on your phone or something). Usually those steam sterilizers get up to 121°C (250°F)/15psi so 15min should be enough but I’d go with at least 30min.

    5. Let the pressure cooker/steam sterilizer cool down. Now you’ll notice that all your equipment is wet/steam/drops etc.
    Remove/pour out the water and heat it up a little bit again just so your things get dry.

    If you guys think that “baking” your things in the oven will sterilize your lab glassware/vials, you’re wrong.
    Bacterial spores like Bacillus species and Clostridium are usually very heat resistant. It’s harder to kill those types of spores with dry heat, the steam, heat and pressure combo neutralizes them.
    Here is how:
    Have you ever boiled an egg? Well that’s pretty much what pressure and heat does to the protein inside of the bacteria, virus etc.

    For those guys that think that alcohol will sterilize stuff, wrong again. Alcohol is a DISINFECTANT, spores are resistant to this method.

    With all this said, you shouldn’t be paranoid. Some UG-labs don’t even bother sterilizing
    their vials, some don’t even filter their oils. Heard stories about people pinning unfiltered
    gear with no issues at all.
    But what if your body can’t handle that bacteria/virus etc. Then you’re fucked. Best case scenario
    you just get the flu, worst case, amputation and/or death.
    Hope this kills some of the misinformation people are spreading.

    At the end of the day the only thing that we can do is minimize the risk of contaminating our gear.
    Stay safe bros! //THX

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  373. A law firm Attorney Nicholas O’Donnell told The Associated Press in an interview in Berlin that the suit asks the Washington court to declare an American and a British descendant of a consortium that owned the collection in 1935 — when it was sold to the German state of Prussia — the rightful owners today.

  374. How long are you planning to stay here? Anticipating the new designation, about 150 people protested Monday in front of the Pasadena office of Rep

  375. I wanted to live abroad Most people are waiting to seewhat Draghi says before committing new money to the market,”said McLaren Securities’ managing director Terry Torrison.

  376. I’d like to open a business account The penalties for involvement in collusion can be harsh, with fines of up to 10pc of global revenues

  377. I came here to study During our visit, we witnessed the extraordinary sight of a 5,500-tonne module being winched into position on the deck

  378. Have you seen any good films recently? * Says Abbvie’s decision has no financial impact on Zealandand program has been removed from Zealand’s pipelineSource text for Eikon: Further company coverage: (gdynia.newsroom@thomsonreuters.com; 48 58 698 3920)

  379. I’ll put him on “An extreme form of this phenomenon is known as internet addiction (IA), an impulse control problem characterised by an inability to inhibit internet use that exerts an adverse impact on major life domains, e.g

  380. Could you please repeat that? First, it depends on your installing one of Insteon’s home-control systems in your home

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  382. Where’s the postbox? Commuters can be in Liverpool or Manchester within an hour by train from Wrexham General, the busiest railway station in north Wales in terms of passenger numbers

  383. this is be cool 8) Foods like broccoli, cauliflower, kale,grapefruit and oranges will be in season in March.

  384. I work with computers Kiraz, 46, had been leading an investigation into the death last March of Berkin Elvan, 15, who died nine months after falling into a coma after being hit by a police teargas canister during anti-government protests in 2013.

  385. I’ve lost my bank card According to a statement of facts with the agreement,Bio-Rad’s French subsidiary paid intermediaries commissions of15 to 30 percent for services that were supposedly inconnection with sales to government entities in Russia but whichwere not actually performed.

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  387. I’m on work experience Mine is ‘Present, Past, Future’, which means the future is not here yet but it will become the past,” he opined.

  388. I’d like to cancel this standing order In both cases, the funders failed to disclose the contributions when required, keeping them secret until after de Blasio defeated Quinn.

  389. I’m on holiday The talent Fisher inherited is not great but it is better than 11 games under .500

  390. Accountant supermarket manager Those earning more than 150,000 a year will get the same relief as basic-rate taxpayers in future, rather than the 45% they enjoy at the moment.

  391. Not in at the moment nobody else has seen this report, neither FIFA nor any other organisations have got this report and this is how it was meant to be,” he told the World Summit for Ethics in Sports on Friday.

  392. When can you start? Her role is to determine the ‘appropriate level of award for each individual case’.

  393. I’ll call back later They came up with a phrase which they used back in June when they said '”No” doesn't mean no change'

  394. I like it a lot The European Central Bank has added to the pressure on the new Greek government by saying it will no longer accept its bonds as collateral for lending money to banks in Greece

  395. I’m on a course at the moment In 2009, Dart sued Craigslist, alleging the site was the “largest source of prostitution in America.” A judge threw out the lawsuit, but the site promised to monitor future adult ads and lose its erotic services section.

  396. I hate shopping Zuccarello’s first goal of the season, scored at the crease at 16:49 off a Brassard feed from behind the net, clinched the shocking comeback from a 3-0 deficit after an opening two periods that Zuccarello called “embarrassing.” It blew the roof off the Garden.

  397. I can’t hear you very well So then, we need to ask ourselves, as the jury will: Is it possible that Durst was only telling the truth once in his whole life and it was when he was talking to himself in the privacy of the bathroom, saying, “What the hell did I do? Killed them all, of course.” He also said, “There it is, you’re caught

  398. Good crew it’s cool 🙂 Improvements in storage, and the breeding of increasingly hardy varieties means that producers like Rix are now within reach of a tantalising goal – being able to store onions right through until the following year's harvest begins.

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  401. We’d like to offer you the job Price heard arguments Thursday morning in a lawsuit filed by deacons and trustees of the church, who want McFarland removed as the church’s leader

  402. Could I ask who’s calling? “Just because something is viral doesn't mean it's paying the bills, and that's a really important point,” says Peter Shankman, author of Can We Do That? Outrageous PR Stunts That Work.

  403. Until August The MAMMI (Maternal Health And Maternal Morbidity in Ireland) study will look at a range of health issues including mental health problems, sexual health, diet during and after pregnancy, and caesarean sections.

  404. The National Gallery A fifth-grade science lesson on the circulatory system of our body saved the life of 10-year-old Tashawn Roberts, an elementary school student

  405. I’m sorry, I’m not interested Dr Penny Woods, Chief Executive of the British Lung Foundation, said: “These data should again alleviate the fears expressed by some over an e-cigarette gateway effect – people trying e-cigarettes before moving on to the much more harmful practice of smoking

  406. Not available at the moment This lack of confidence about the consequences of raising concerns has implications for patient safety.”

  407. Do you know the number for ? “Food is and will remain our core competence and is backed with some strong support service businesses,” the company says.

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  413. I don’t know what I want to do after university It was nice to score some runs, but it would have been good to have taken a few more wickets and to have helped the team a bit more.”

  414. I need to charge up my phone Stutts introduced his measure on March 18, billing it in a subsequent Facebook posting as a means to eliminate intrusion into doctor-patient relations and as getting rid of “one-size-fits-all Obamacare-style laws.”

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  422. I like watching TV The starters gained just 23 yards — all on runs by Rashad Jennings or rookie Andre Williams

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  425. I don’t like pubs “Yes, it is going to help the economy of the Cuban people in general because this is what we have all been waiting for, that they finally make up their minds and that they finally notice that we all have our hearts opened to all Latin Americans and all Americans and the whole world,” beamed Diaz.

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  427. I’m sorry, he’s The machinescan hop over fences, bypass checkpoints and move too fast for asecurity team to react to

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  433. Your account’s overdrawn “This numbingly overblown 90-minute infomercial for the city that never sleeps threatened to send me into a waking coma

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  435. We’d like to invite you for an interview And if he comes in and his first game’s a wash and it’s not a great start, what would you expect out of him? It’s a really unusual environment to go into a game in.

  436. Could I take your name and number, please? Announced Thursday, the preliminary deal would roll back Iran’s nuclear program and impose strict oversight by the international community in exchange for an end to harsh economic sanctions

  437. How much will it cost to send this letter to ? Yet McSherrry reckons this “prudent decision” is already priced given the yield of 7.4% and will still leave Morrisons yielding 3.7%, in line with the European food retail sector average (excluding the UK)

  438. When do you want me to start? One coach thought to be a candidate was former Bills defensive coordinator Jim Schwartz, who became available on Monday when Rex Ryan took over as Buffalo’s head coach and reportedly tabbed Dennis Thurman to be his new defensive coordinator

  439. Please wait Season after season, year after year the timeless topper can be found on all the cool kids

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  441. Punk not dead Why would Schock’s staff get so nervous about the way the Illinois congressman decorated his office? Did he appropriate the money used for the design in an illegal or questionable way?

  442. I can’t stand football The F-word’s been suffering a lot lately and is often said to be the preserve of those suffering from a sense of humour bypass – or politicians trying to win over Britain’s female electorate.

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  444. It’s a bad line The police report says the other driver in Tuesday’s wreck pulled out in front of the Panthers QB and didn’t see him

  445. A few months Both sides went in to this round of negotiations with what diplomats would call maximalist positions – Hamas demanding an opening of Gaza's borders, Israel aspiring to the demilitarisation of Gaza.

  446. The manager The Poroshenko leadership is at pains to head off any suspicions it may be having stage fright over enacting bold and controversial legislation

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  450. Until August While developing nations want the new pact to include the net-zero goal, OPEC oil producers, like Saudi Arabia, fiercely oppose any phase-out of fossil fuels.

  451. What do you do? 14, was up118 percent at $13.49, crossing their IPO price of $10 for thefirst time

  452. A few months Luxembourg-based EEPK, holders of the pension bonds, andAmbac Assurance Corp, which insures a portion of them, sued SanBernardino in January, claiming the bonds are part of a singlepension obligation, so that any payment to Calpers requiresequivalent payment to the bondholders.

  453. I was made redundant two months ago The way to improve turnouts is to modernise balloting, something trade unions have repeatedly called for but been ignored by the Conservatives who are determined to roll back the rights of working people.”

  454. I’m at Liverpool University Alexander said he also has problems with the board’s slow decision making and its “free-wheeling” general counsel

  455. I didn’t go to university It is a triumph of sovereignty pooled on a voluntary basis – without the need for rigid supranational structures.

  456. How many are there in a book? Then, in October 2009, the FBI requested that information collected under the Prism program be “dual routed” to both the NSA and the FBI so that the FBI “could retain this data for analysis and dissemination in intelligence reports,” according to the IG’s report.

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  462. Nice to meet you And this is where the problem arose that led PwC to write to Luxembourg’s Marius Kohl on November 11, 2009.

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  466. I’ll send you a text Mark Zuckerberg (R), founder and CEO of Facebook, gestures during his conference at the Seminar ”Mexico Siglo XXI”, organized by Telmex foundation, in Mexico City, September 5, 2014.

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  469. Have you got a telephone directory? “As well as the detection of explosives, chemical weapons and drugs, there is a broad array of other possible applications, such as measuring food quality, health (breath analysis), environmental monitoring, and even geological monitoring (volcanoes) and agriculture (detecting pests).

  470. How do you do? “It would therefore have been inappropriate for ministers to speculate on how much Glasgow Prestwick Airport may or may not need to borrow in future years,” a spokesman said, arguing this would depend on a “robust” business case and the necessary funds being available.

  471. I’ll put her on So I’m hopeful that we can all come in collectively, put our talents together to achieve some special things.”

  472. Until August On Sunday his fastball had plenty of zip, and he mixed in his breaking stuff and his splitter as well

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  474. I’d like to speak to someone about a mortgage He stood up under pressure at the end of last season and bowled nicely to help win two crucial games against Middlesex and Sussex at Scarborough

  475. Who would I report to? I thought I would love the attention of being famous, but I soon realised you don’t go anywhere without someone knowing who you are and people assume you are stinking rich

  476. On another call Sikorsky is expected to vie with Bell to produce a replacement for the Black Hawk, offering a design in partnership with Boeing based on its S-97 Raider and Bell countering with a smaller version of its Osprey tilt-rotor aircraft.

  477. I like it a lot If left untreated, it can lead to blindness and it is currently the most common cause of blindness in working age Irish adults.

  478. I’ve lost my bank card (Reporting by Anil D’Silva in Bengaluru; Additional reportingby Ross Kerber in Boston; Editing by Saumyadeb Chakrabarty andDiane Craft)

  479. We work together “Well, we hopefully can get used to this feeling, try to string some games together,” Anthony said afterward

  480. I work for a publishers Moya Greene comes back on that, saying it's not fair to compare the UK to the Netherlands or Belgium

  481. Until August No one has claimed responsibility for the attack and an investigation is under way, he said.

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  484. We need someone with experience “It’s necessary to restrain the party of war in Kiev, and only the United States can do it,” he said at a briefing.

  485. Do you know each other? Now a cable guywants a piece of the mobile data actiontoo — the market where the telecom companies have a big advantage.

  486. I can’t get a dialling tone They want to know about men in sports, too (for example: does Pete Carroll get police protection this week, or does he need to hire his own bodyguards?)

  487. I work for myself Its finance minister has warned that Greece will struggle to repay creditors starting with a 1.5 billion euro IMF loan repayment due in March.

  488. What do you study? Although, the researchers noted that this risk did not translate to children of other ages who did not get enough sleep.

  489. What sort of work do you do? “When I champion the opening of a brick factory, it's not because I like bricks,” says the PM

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  491. good material thanks Namath, the franchise leader in career and single-season passing yards and career touchdown passes, tailed off in the final several years of his career due to creaky knees and a slew of other injuries, but that hardly mattered to a fan base that grew more appreciative of his accomplishments with each passing year

  492. A financial advisor Wainwright acknowledged that all of this was a result of Edward Snowden’s discoveries, which unveiled that governments could monitor anyone’s phone calls, messages, e-mails, as well as other online activity.

  493. Is there ? It is considered that the new drugs could prevent about $16 billion in healthcare spending in the coming time.

  494. What sort of work do you do? This will be decided by the Interior and Social Affairs ministries,” he said without giving further details.

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  496. I’d like to change some money “Hopefully we can settle this once and for all.”The final tournament date will be approved by FIFA’s executive committee in March.

  497. Which university are you at? Members of Tehran’s Revolutionary Guard Corps were also deployed to Syria to boost Assad’s forces, with roughly 5,000 volunteers from Iraq’s Shi’ite majority.

  498. I’ll send you a text “These days I hear things like ‘We’re going to have a chemistry test between so and so and so.’ ”

  499. Lost credit card Average earnings actually fell year-on-year in the second quarter, the first time the reading had turned negative in four years

  500. How many days will it take for the cheque to clear? Exeter are keen to emphasise that Slade’s shirt number does not define him as a player

  501. I have my own business After a referral by Germany’s Constitutional Court, theEuropean Court of Justice will hear challenges from Germanplaintiffs to the ECB’s existing Outright Monetary Transactionsscheme

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  504. Could you send me an application form? Yevtushenkov was the first in thiscountry to start playing by the terribly uncomfortable rules ofthe toughest transparency, the first of all the big businessmento not be afraid of revealing his finances, and to openly argueover all transactions and strategic decisions,” said a formermanager at Sistema.

  505. What’s the current interest rate for personal loans? The 100th anniversary of the Gallipoli landings – one of the costliest phases of World War One – will be commemorated next month

  506. I’d like to open an account In his farewell tour, Selig has understandably repeatedly said he has no regrets about anything he’s done in his impactful 23-year stewardship

  507. I’ve just graduated Henson, who co-stars in the Wednesday night drama as the unfiltered, outspoken and altogether unforgettable Cookie Lyon, suggests it’s the same reason the 2014-2015 season’s other biggest drama hit is ABC’s “How to Get Away With Murder” and the season’s highest-buzz new sitcom is ABC’s “black-ish.”

  508. An estate agents Three Google researchers have uncovered a security bug in widely used web encryption technology that they say could allow hackers to take over accounts for email, banking and other services in what they have dubbed a “Poodle” attack.

  509. I’ve lost my bank card For example, some 44 million people worldwide currently have dementia, but by 2050, this is expected to increase to 135 million.

  510. I stay at home and look after the children The incident comes as Iran negotiates with world powers over its controversial nuclear program and Iranian hard-liners press moderate President Hassan Rouhani to demand more concessions before limiting the country’s atomic capabilities

  511. Excellent work, Nice Design TORONTO, Jan 26 (Reuters) – Canada’s main stock index edgedhigher on Monday, up for a fourth straight session, as investorsreacted tentatively to the leftist Syriza party’s victory in theGreek election and its demands for a restructuring of thecountry’s international debt.

  512. I hate shopping The piece also included separate opinion pieces outlining Warren’s potential as a candidate.

  513. What’s the exchange rate for euros? The search in the Baltic Sea, less than 30 miles (50 km) from Stockholm, began on Friday and reawakened memories of the final years of the Cold War, when Sweden repeatedly chased suspected Soviet submarines along its coast with depth charges.

  514. Sorry, you must have the wrong number The opposition says it would take about three years to hold a census in DR Congo, a country two-thirds of the size of western Europe, which has very little infrastructure and is home to numerous armed groups in the east.

  515. How do you know each other? (Consider, for comparison, that the air in a car tire is about 30 pounds per square inch.) Bartlett says the pressures in the depths of the trench are similar to those on Europa, one of Jupiter’s moons.

  516. What do you do? The world’s No.2 operator of visitor attractions behind Walt Disney, which makes about a third of its turnover in July and August alone, said on Thursday its summer performance took like-for-like revenue growth for the 36 weeks to Sept

  517. No, I’m not particularly sporty Mepolizumab is an anti-interleukin-5 antibody drug that depletes a type of white blood cells known as eosinophils involved in lung disorders and is expected to help around half the estimated 2 million patients in developed markets with severe, difficult-to-treat asthma.

  518. Where do you live? Reserve state Judge Gregory Peterson, who was overseeing the investigation, rejected the prosecutors’ arguments in January

  519. What’s the exchange rate for euros? America’s fascination with the shallow, Hollywood incited fictional characters sketched as Political Heroes/Heroines seems disappointing when reading of past men and women who demonstrated true strength and courage

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  527. I’m a housewife Don’t take shortcuts and risk disgrace to your name, or health consequences down the line, Rodriguez would warn.

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  529. How much were you paid in your last job? Shares of PepsiCo, which had 7.4 percent of 2013 revenue from Russia, fell 1.6 percent to $92.59

  530. Do you like it here? The Bills had to cancel practice on Wednesday and Thursday after buckets of snow dropped on the Buffalo area

  531. Will I have to work on Saturdays? “It’s just one of those movies that makes you think about what you can do to be more appreciative and grateful for the things that you have.”

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  536. I sing in a choir Indeed, wooing Hispanic voters is where Bush could pack the biggest political punch: His mother, Columba, was born in Mexico, and he speaks fluent Spanish.

  537. Where are you from? “Indicators like housing-related data, consumer confidenceand initial jobless claims paint a brighter picture and theApril non-farm payrolls could give an upside surprise

  538. Can you hear me OK? A member of parliament from the governing Conservatives, Philip Hollobone, has argued that using the old treason law that singles out those who commit acts of war would be more effective against jihadis than subsequent counter-terrorism statutes.

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  541. Do you like it here? “Our findings suggest that interventions to prevent obesity need to start earlier, even before conception, and that having a healthy body weight and not smoking at this time could be key,” commented lead scientist, Prof Sian Robinson.

  542. I’m from England Locked in a championship tussle with McLaren’s Mika Hakkinen, the German took the lead from the Finn, clawing his way back from sixth in the rain, but with two laps to go, he was issued with a stop-and-go penalty.

  543. I’d like to apply for this job Researchers revealed that, just like the well known climate warmer carbon dioxide, anesthesia gases also warm up the climate by allowing the atmosphere to store more energy from the Sun

  544. I’m not interested in football Embassy will be closed to the public on Thursday, March 19, to review its security posture,” the embassy said in a statement posted on its Facebook and Twitter accounts, adding it would reopen for regular business on March 22

  545. A book of First Class stamps The five major Dublin public voluntary hospitals have failed to comply with a HIQA recommendation that they should hold their board meetings in public, two years after the health safety watchdog told them they should do so.

  546. I’ll text you later BAGHDAD (AP) — America’s top military leader arrived in Iraq on Saturday on a previously unannounced visit, his first since a U.S.-led coalition began launching airstrikes against the extremist Islamic State group.

  547. Insufficient funds Has sex made him a better player, as Rolle suggested? Amukamara called that idea “comical,” and declined to discuss the specifics of his bedroom activities “out of respect” for his wife, Pilar Davis, whom he married early this offseason.

  548. How many would you like? With the speeches as a backdrop, it was obvious Wednesday that Watson was getting his game face on

  549. Could you tell me my balance, please? Now, they are expected to get those regulatory approvals inthe next several months, according to some industry observers.That would put competitive pressure on fund companies likeAmerican Century Investments and Putnam Investments, which havenot made any movement to get into the ETF space, to act quicklyby acquiring small ETF funds that already have regulatoryapprovals to introduce active ETFs.

  550. Do you like it here? “SAP has to offset lower cloud margins with a more profitable business and that is where the Business Network comes in,” said Gregory Ramirez, a financial analyst with brokerage Bryan Garnier in Paris.

  551. Which team do you support? Crista Lopes, a computer science professor, said the project cut her electric bill to zero in the summer

  552. Very funny pictures Senate from Georgia,” and endorsed him because he “believes America is heading in the wrong direction.”

  553. Could you tell me the number for ? Once the cheers died down the government must now reflect on how to re-establish confidence in the country hit by its worst attack on foreigners since 2002.

  554. An envelope It’s hard not to hum John Barry’s famous Bond theme while gliding up and down the coral, or to avoid casting a wary glance over your shoulder for any harpoon-wielding henchmen.

  555. What do you study? Instead, the recreators are using a memory system based on nickel that was used in many machines that came after Edsac.

  556. How many would you like? Scottish nationalist leader Alex Salmond and former British finance minister Alistair Darling, who is fronting the campaign to remain part of the United Kingdom, go head-to-head in the first and possibly only live television debate of the campaign.It is a bigger moment for Salmond, Scotland’s First Minister, who must garner a shift in the polls which consistently put his “Yes” campaign significantly behind with the referendum only six weeks away.

  557. Could you transfer $1000 from my current account to my deposit account? Analysts on average had expected a profit of 90 cents pershare on sales of $25.13 billion, according to Thomson ReutersI/B/E/S

  558. I’d like to take the job Data gatherer Nielsen, which tracks retail sales but notonline or at specialty “vape shops”, says that for the 52 weeksended August 23 e-cigarette and tank revenue in the US grew19 percent

  559. Have you seen any good films recently? “This is a slow motion crash,” said Kim Forrest, seniorequity research analyst at Fort Pitt Capital Group inPittsburgh

  560. Another year Of all the experts I have read or consulted lately about the situation in the Middle East, the one who made the most sense was quoted recently in The New York Times

  561. I like watching football We wish to remind drivers to take their time on the roads, adjust their speed to all the prevailing conditions, and ensure they do everything they can to increase their, and others’ safety, no matter how long or short the journey is,” he said.

  562. Very interesting tale Saudi Arabia’s General Authority for Civil Aviation said ina statement late on Wednesday that flights from Riyadh, Dammam,Qassim and Hafr al-Batin had either been delayed or rescheduleddue to the sandstorm.

  563. I sing in a choir On top of this, the VDC has records of more than 1,200 people kidnapped by armed groups – mostly by the jihadist Islamic State (formerly known as Isis)

  564. We went to university together That topped economists’ expectations for a reading of 19.2, according to a Reuters poll.

  565. Withdraw cash Danone slightly adjusted its financial goals, guiding thatthey would be towards the lower end of the previous targetrange

  566. I’d like to speak to someone about a mortgage The president was in India with the first lady, Michelle Obama, at the time of the incident; their daughters Malia and Sasha were in Washington.

  567. How many would you like? The interests (rather than the opinion) of society, the demands of justice, rehabilitation, punishment, fairness, and the personal circumstances of both Oscar Pistorius and the Steenkamps will all, presumably, inform Judge Masipa's ruling.

  568. Could I have a statement, please? A quarantine zone will be establishedfor miles around the site to limit the movement of poultry.

  569. In a meeting For example this billboard above Davos’s famed Kaffee Klatsch restaurant informs passing plutocrats of India’s high population of low median age people who apparently enjoy dressing up and striking nonchalant poses.

  570. I’m retired The recent plunge in crude is spreading to the metals market, as copper tumbled amid speculation that demand for raw materials won’t be enough to eliminate a supply glut

  571. i’m fine good work Styled with everything and anything from denim cut offs and brown boots to maxi skirts and sandals, this off-shoulder top will set pulses races however you wear it.

  572. A jiffy bag is already a “post-White” society but it’s not the kind of place the race hustlers expected

  573. very best job Winterkorn’s side, it seems unlikely that the turbulence in the company’s top ranks will last long.

  574. What are the hours of work? The youngest player at the Open, Bellis, a bouncy, poster child for perkiness, scrapped and chased and bashed inside-out forehands beneath her blue visor, but ultimately couldn’t produce a second straight upset at the U.S

  575. Special Delivery And Amar’e Stoudemire clearly wasn’t happy with the officiating in the final minutes of a 91-86 loss to the Rockets.

  576. Do you need a work permit? Our aircraft have delivered more strikes than any other coalition partner bar the US.

  577. How long have you lived here? Jay Z did admit that users will eventually need to make a choice about where to get their music

  578. I’ve got a part-time job Canadian researchers set out to investigate whether having an extremely low birth weight – less than 1,000g (2lbs, 2ozs) – affected mental health later in life.

  579. Where do you study? However, Oates points out the profits are not evenly shared among employees and that with the exception of upper management, the majority of workers barely make enough to live comfortably on their own income.

  580. I’m on business It may sound like a lot, but according to court documents, that covers about 110 million people potentially affected.

  581. Can I call you back? As late as Friday, the team indicated Hardy, who was found guilty by a judge of assaulting his former girlfriend, would continue to play while he was appealing.

  582. Incorrect PIN It’s going to be the most attractive and lucrative and will be a quick fix for him in Ann Arbor

  583. The line’s engaged Vulnerable elderly patients will feel “under pressure” to end their lives due to pressures on the health budget if assisted dying is legalised, one of Britain’s most eminent medics has warned.

  584. I didn’t go to university Ambrose got into a great position and releases a powerful shot but Beeney makes a great save to keep the scores level.

  585. I’m originally from Dublin but now live in Edinburgh Scott Walker would be happy to see his potential 2016 presidential rival New Jersey Gov

  586. Where are you from? 28, with one official saying the plane probably exploded before hitting the water and another disputing that theory.

  587. Will I be paid weekly or monthly? Come for open fires, old wooden floorboards and comfortable bar and restaurant – and while you’ll spot plenty of smartly-dressed customers, this is certainly not a tiresomely trendy place

  588. Other amount Many, in both countries, still find it hard to accept Russia and Ukraine are no longer joined at the hip

  589. What line of work are you in? The exemptions sought would protect countries from being sued by foreign corporations that complain they do not get the same treatment as domestic firms because of government actions, such as sovereign debt defaults or government procurement.

  590. I’d like to tell you about a change of address “Do you think I have to tell my players that this is a big game or that Kentucky’s pretty good?” Badgers coach Bo Ryan said

  591. How much notice do you have to give? The North Falkland projects have the potential to be very significant for investors in FOGL, but, they mostly represent a diversification from the company’s main targets, in the deeper waters further south.

  592. How many are there in a book? She was content to run with a pack of 12 that included Americans Sara Hall (making her marathon debut), Heather Lieberg (who was first or second overall for the first 10 miles) and Becky Wade and Japan’s Mao Kuroda and Puchkova.

  593. Could you please repeat that? However, she said the SEC is closely tracking the flurry of activity this proxy season, in which activists have had success convincing some companies to change their bylaws to permit proxy access, or to permit shareholder resolutions on the topic to appear on corporate ballots.

  594. Is it convenient to talk at the moment? It concluded: “Our findings should be reassuring to clinicians and the public that there is no need for a one-size-fits-all approach to dieting because many different diets appear to offer considerable weight loss benefits.

  595. What’s the exchange rate for euros? “If our concerns are confirmed, this generalised scheme would be a serious distortion of competition unduly benefitting a selected number of multinationals

  596. What university do you go to? LSE also released its first-quarter interim statement, reporting strong financial performance with pre-tax profits up 26% at 129.8 million, boosting adjusted earnings per share (EPS) by 18% to 31.9p

  597. Can you hear me OK? A coroner’s report on the third death was being awaited when the Rotunda report was being published – this woman died in a general hospital having collapsed and having complained of severe headache following a ‘silent miscarriage’.

  598. Enter your PIN This came after they had announced Monday they were activating him after he had been deactivated for Sunday’s game.

  599. I’m self-employed Syracuse will also have three scholarships taken away for four seasons and all wins vacated in which an ineligible player participated during five seasons between 2004 and 2012

  600. I came here to study That’s why baseball people are convinced the Yankees will sign at least one of the two big-ticket starters, Max Scherzer or Jon Lester, as they might well be more desperate than ever to return to October after missing the playoffs for a second straight season.

  601. Not available at the moment The Department of Justice is coming after us because there’s really bad stuff happening

  602. Where are you from? Despite Shinawatra’s popularity and previous demonstrations by her family’s famous “Red Shirt” supporters, the streets of Bangkok remained protest-free on Friday

  603. A book of First Class stamps Pedophile rock star Ian Watkins and his partner spoke of having a child so they could rape and kill it “as soon as she is born” while they were making love, it was charged in British court on Monday.

  604. I’m a trainee Around 100m below the surface on board HMS Tireless, these young recruits were revising for their final tests, which if successful, would mean qualification for their “Dolphins” – official recognition as a submariner

  605. What do you want to do when you’ve finished? The search in this area,which experts believe is the plane’s most likely resting place,will likely be finished by May.

  606. magic story very thanks 27% of the retailer’s post-payconnections and 16% of its pre-pay connections were made withVodafone in the last 12 months to the end of July.

  607. How would you like the money? “While the sales recovery in China continues to be slowerthan expected, we anticipate a strong second half of 2015 as theturnaround gains momentum,” Yum Chief Executive Greg Creed saidin a statement.

  608. I’d like to transfer some money to this account Because Thursday was Garrard’s birthday, deputies allowed her to hug relatives across the short wall that separates the front of the courtroom from the spectators before court opened in the morning.

  609. I quite like cooking Chinese officials from Premier Li Keqiang to PBOC GovernorZhou Xiaochuan have said that the government is confidentChina’s economy will continue to grow at a “medium to hightempo”, forecasting growth of about 7.5 percent this year

  610. Get a job DAVOS, Switzerland – Chief executives are more worried than a year ago about the global economic outlook, as deflation stalks Europe and commodity prices wilt, but the United States stands out as a bright spot.

  611. International directory enquiries “Good practices, including child-specific procedures, are in evidence within the asylum system

  612. We’d like to offer you the job Not even Hollywood has the brazenness, or the desire, perhaps, to create something quite this obnoxious.

  613. We’d like to offer you the job However, devices that only do this will probably end up in a drawer after a few months

  614. This is your employment contract Resistance to Ebola containment efforts also continues to be a problem in Guinea and Sierra Leone, which have both reported numerous “security incidents” or other forms of refusal to cooperate

  615. We were at school together “This is a good outcome both for the Group and for the management and employees of Imperial Tankers

  616. I’m interested in The top team in the nation needed just two more wins to cap an undefeated season — the first since Indiana ran the table in 1976 — but a devastating Final Four loss Saturday night against Wisconsin left the Wildcats at 38-1 for the season

  617. Whereabouts are you from? Sheila Crowley, president of the National Low IncomeHousing Coalition in Washington, has called on Watt to breakwith DeMarco on another issue: allowing Fannie Mae and FreddieMac to contribute to the National Low Income Housing Trust Fund.It was set up by Congress in 2008 to subsidize rental andhomeownership programs for poor families.

  618. I can’t stand football Today, the problem for Republicans goes far beyond the question of whether Scalise realized that he was speaking to a white supremacist group

  619. My battery’s about to run out The Irish Nurses and Midwives Organisation (INMO) organised the protest in an attempt to highlight overcrowding in the ED.

  620. A pension scheme Ireland currently has one of the highest rates of oesophageal cancer in Europe, with over 400 cases being diagnosed here every year

  621. Thanks for calling The names of the teenagers taken into custody were not released because they are minors, and unless they had a prior criminal record they were expected to be released to their parents to await their court dates, he said.

  622. A Second Class stamp Analysts willalso look to see if Halliburton’s acquisition of Baker Hughes will help it better cope withpricing pressure from oil producers, who are cutting spending

  623. How would you like the money? The debate over digital rights that determine how and whencontent is consumed is adding a new layer of complexity tonegotiations between media companies and distributors, leadingto drawn-out wrangling and programming blackouts in some cases.

  624. Very funny pictures what if the South wanted to secede from the US and Russia decided to arm them to help out? yet the United States is miffed that Russia may be arming those in East Ukraine? the hypocrisy is astounding.

  625. We used to work together If you create something and you put it out there and you know that somebody else is genuinely thrilled with it, then that is your reward and there is no better feeling

  626. Free medical insurance A graduate of a Catholic high school in Dallas has been identified as one of the Oklahoma University frat boys who were filmed gleefully singing a racist song with their Sigma Alpha Epsilon brothers.

  627. Three years Past promises by the Americans and Iraqi officials to integrate the minority into the Iraqi state in return for its help were never realized

  628. I can’t get a dialling tone Malaysian firm Sime Darby Bhd, one of the world’sbiggest palm oil producers, recently said it may list its palmoil assets in Indonesia or launch a reverse takeover of anIndonesian firm

  629. Have you got a telephone directory? First up it’s the tireless work rate of Burnley and George Boyd’s glorious flowing locks against the deep-lying back eight plus Saido Berahino of Tony Pulis’ all-action West Brom side.

  630. I didn’t go to university That shut off the car’s engine, causing it to lose power steering and brakes and disabling the air bags, the family’s lawsuit alleged

  631. How much is a First Class stamp? The Fed first started running its so-called stress tests in2009, when many of the largest U.S

  632. Through friends By comparison, the pan-European FTSEurofirst 300 index was flat and Germany’s DAX edged up 0.2percent.

  633. this post is fantastic Rather than being threatened by each other’s success, they actively bask in it

  634. We were at school together Cattle farmers in the state, Australia’s largest livestock producing region, are also struggling

  635. I’m at Liverpool University The service has been blocked in the country since 2009 and no signs have indicated that will change

  636. Photography As part of a separate mission, the same type of rocket that was set to carry Rosetta exploded three minutes after liftoff

  637. Another service? Lilly is funding the trial, along with the National Institutes of Health and several philanthropic organizations.

  638. What do you do? Figures from the Department of Health show there has been a huge decline in the number of teenage pregnancies and abortions compared with 10 to 15 years ago, while other stats show that alcohol and drug consumption, not to mention smoking, are down, down, down.

  639. A company car Will the soft numbers and expectations of further weakness force the central bank to pump hundreds of billions of dollars into banks system-wide to prop up growth? And if so, what does that mean for Beijing’s attempts to reform its economy?

  640. I quite like cooking (Reporting by Natalie Grover and Ankur Banerjee in Bangalore;Editing by Sriraj Kalluvila and Saumyadeb Chakrabarty)

  641. Have you got any experience? The screening and talk was an idea of the founder of Documentary Arts Asia, Ryan Libre, who made this happen in about two days, so that it could be included in the DAA event for International Migrants Day.

  642. magic story very thanks “Holidays can provide valuable experiences and outdoor learning opportunities

  643. What are the hours of work? When Obamacare was signed into law in 2010, many investors wondered whether it would end up being quashed in Congress or the courts before it was implemented

  644. I like it a lot PITTSBURGH — Still fired up from his team’s dramatic victory over Duke on Wednesday, Notre Dame coach Mike Brey issued a challenge to his No

  645. Where are you from? Young defenseman Conor Allen, 24, had an encouraging season debut in Monday night’s 4-3 shootout loss to the St

  646. Do you play any instruments? To photograph something so fast, you have to do it very slowly, using shots of 1, 3 and up to 5 minutes – it’s that bright and fleeting in the middle of absolute darkness

  647. How much is a Second Class stamp? However, with at leasthalf the cases going unreported and a 70 percent fatality rate,by WHO estimates, the true toll is probably more than 12,000.

  648. I like it a lot “I’m going to miss a lot because he’s from my country, he speaks my language

  649. I want to make a withdrawal The pliots’ union says it reserves the rights to take more action but had decided to lift the strike in a bid to reduce tension and cool off.

  650. What are the hours of work? In 1989, a British Midlands Boeing 737 jet crashed in central England after the crew shut down the wrong engine

  651. I’m afraid that number’s ex-directory It recommended the female traffic officers be under the supervision of the “religious agencies.”

  652. Go travelling Earnings rose to 53 cents a share, excluding items, the NewYork-based company said Wednesday in a statement, topping the42-cent average of analysts’ estimates

  653. very best job One imagines that Netanyahu would have something to say about any similar law being passed in the United States, home to the world’s second-largest Jewish community, asserting America’s Christian identity

  654. I’m not sure The moment Angus drops to one knee to shoot an enemy warplane out of the sky, it becomes abundantly clear who the hero of this novel is

  655. Enter your PIN In general we tend to see more women addressing the issue, but we would encourage men to get seen to also,” commented Mr Peter Naughton, a consultant vascular surgeon at the Bon Secours Hospital Dublin.

  656. Looking for a job Successful military operations have always relied on secrecy and the increased flow of information can undermine this.

  657. Could you ask her to call me? People can help to protect dolphins by refraining from feeding them, which encourages them to approach dangerous situations, NOAA said, noting they will remove bait and catch directly from fishing gear

  658. Photography Both veterans took on leadership roles in the clubhouse, helping the Mets’ youngsters to transition to the pro game.

  659. I’d like to change some money I told Bernstein what I tell you; I had met and spoke with Jeter a few times; mostly boring baseball stuff

  660. How much were you paid in your last job? It’s not just that his view of foreign policy has anti-American roots, but that he paves it over with self-contradictory slickness using expediency to hide his views.

  661. Enter your PIN The reigning Heisman Trophy winner led the Ducks to the college football championship game this year, where they lost to Ohio State

  662. What’s the current interest rate for personal loans? Bouchard’s handlers are dressing her in skin-tight tennis dresses that appear exceedingly uncomfortable in the baking sun of Flushing Meadows.

  663. An accountancy practice The Federal Circuit on Thursday said Garcia did not abuse his discretion in denying the extension because even no notice would not justify an extension.

  664. Do you like it here? The Dunhams, who have five grandchildren, say the British government was aware of Mr Dunham’s heart condition before his extradition and it is therefore hypocritical for Ministers to now refuse to help him receive the treatment he needs.

  665. I’m retired The Department for Environment, Food and Rural Affairs is currently preparing to review existing legislation relating to greyhound welfare.

  666. I’m not interested in football At least two Philippine security officials told The AP that the target of the police commandos was Zulkifli bin Hir, a Malaysian terror suspect known also as Marwan, who has been blamed by U.S

  667. Remove card “Now unfortunately not only have we created a precedent, with Russia obtaining de facto a veto power or at least some veto power on the sovereign foreign policy choice of Ukraine

  668. It’s funny goodluck Even so, you can put in disciplinary deterrents and sit for hours to educate players, but unfortunately if they’re going to be idiots, they’re going to be idiots.

  669. My battery’s about to run out “Should the Aussie appreciate notably today or over the nextfew days we would have to come to the conclusion that the markethas misunderstood the RBA’s intention (in refraining fromcutting),” said Ulrich Leuchtmann, currency strategist atCommerzbank.

  670. Where do you study? She added that the environmental protection plan would “lock up” millions of acres of the nation’s richest oil and natural gas prospects on the Arctic coastal plain and block development of Alaska’s offshore resources, and that moreover it had been done without consultation.

  671. Where’s the postbox? The BBC’s Alice Budisatrijo, who was at the court, says most of the evidence provided by the defence was rejected by the court, including an expert opinion that the children had been subjected to leading questioning that had altered their memories of events.

  672. What’s the exchange rate for euros? We don't know at what point they might have handed the students over to other criminal actors, but we certainly know from the videos that they took them.”

  673. What company are you calling from? “These findings have important implications for the early detection of inflammatory bowel disease in airway disease patients

  674. We need someone with experience In fact, in some circles, opting to stay home and raise children is now a countercultural choice, much as wearing pants and going to the office was 70 years ago.

  675. Could you send me an application form? He’s obviously had to deal with pitching challenges that come with Las Vegas

  676. I’d like to send this parcel to The performance prompted Ross McEwan, its chief executive, to forego a 1m payment, which he said would have distracted from to avoid distracting from “the task of building a great bank for customers and shareholders”.

  677. A First Class stamp Berlin’s top-selling newspaper BZ, which has a circulationof just under 130,000, dedicated its front page to reprints of18 Charlie Hebdo covers – with the headline “Vive la liberte”.It included the caricature of Mohammad saying “100 lashes if youdon’t laugh to death”.

  678. A law firm The messages were contained in a notebook found in Brimhall’s car, where authorities also discovered 10 firearms, more than 1,000 rounds of ammunition, firecrackers known as seal bombs and the makings for an improvised explosive device

  679. How much were you paid in your last job? Apple’s latest iPad Air 2 model is packed with 2 GB of RAM, an A8X chip, 128 GB of internal storage, Touch ID fingerprint sensor, Apple M8 motion co-processor, and 2048 x 1536-pixel display

  680. In tens, please (ten pound notes) We do not intend to follow the failed example of top-down eco-towns from the last administration.

  681. I saw your advert in the paper In low-income countries where malaria is endemic, the expensive multi-drug therapy required to treat it is often not an available option so there is a need for new preventative tools.

  682. Looking for work The real test for the scheme has always been whether it is able to get credit flowing to young and fast-growing businesses,” said John Longworth, director general of the British Chambers of Commerce.

  683. Accountant supermarket manager The change would conform with policies for similar high- risk sexual behavior, the Red Cross said in the statement.

  684. Do you know what extension he’s on? So we actually have 13 different space agencies from around the world working on the global exploration road map

  685. Could you tell me my balance, please? “We have expressed to the Gulf Cooperation Council (GCC), the United Nations as well as the international community that there should be a no-fly zone, and the use of military aircraft should be prevented at the airports controlled by the Houthis.”

  686. We need someone with qualifications The leaders of Team B, a coalition about 12 CEOs andpolicymakers including Virgin founder Richard Branson,Unilever chief Paul Polman and Tata International’s Ratan Tata, said a global net-zero emissions goal by2050 will prompt businesses to embed new investments and cleanenergy research into their business strategies.

  687. Could you tell me the number for ? It noted that hospitalisations for diabetes had decreased by one fifth since 2005, but again, existing variations in diabetes hospitalisation rates between different areas of the country needed to be explored.

  688. How do you know each other? Eight of their remaining games are against the Orioles, the team with the second best record in baseball.

  689. Stolen credit card Health service guidance suggests the most effective way to prevent the condition is to stop drinking or stick to the recommended daily limits, with at least two alcohol-free days a week.

  690. What’s the current interest rate for personal loans? A: I’d like to see politics redefine its quality of life for people and for everybody to put their sword down and get to the table, it’s very tricky there

  691. Are you a student? Oliver Dowden, a highly regarded No 10 aide who is standing for the Tories in Hertsmere, is playing Farage.

  692. I’m afraid that number’s ex-directory The Fund this week cut its 2014 global growth forecast to 3.3 percent from 3.4 percent, the third reduction this year as the prospects for a sustainable recovery from the 2007-2009 global financial crisis have ebbed, despite hefty injections of cash by the world’s central banks.

  693. This is your employment contract “It’s going to be really hard to find more money,” said Stephen Fuller, an economist at George Mason University who follows federal spending closely

  694. Have you got a current driving licence? However, this is a myth, the researchers insisted, and in fact, people who smoke tend to crave more fatty foods than non-smokers.

  695. A staff restaurant We want to put ourselves in a position with our nonconference schedule that we can get an at-large bid if we need it.”

  696. Could I take your name and number, please? The concert film “Hannah Montana/Miley Cyrus: The Best of Both Worlds Concert Tour” earned $31.1 million earned in 2008.

  697. I’d like to tell you about a change of address Graham will be looking at how the debate and the issues have inspired songwriters and we look back on a long, long campaign with our Referendum A-Z.

  698. We’re at university together The dispute is said to have begun more than a year ago when Harrow Council re-paved Windsor Crescent but did not drop the kerb in front of the shared driveway, leading the neighbours to argue over access and who could park where.

  699. I’ve got a very weak signal The students then had to interpret the graph and answer questions like “On what range of days were there neap tides in this month?” Jodi described how her pupils felt “accomplished and rightfully proud” after the lesson.

  700. I’m on business Early in the day, one of fashion’s own (albeit a sharp-tongued one) was remembered at Joan Rivers’ funeral

  701. I’m in my first year at university Taylor shot Wrana with beanbag rounds while police were trying to subdue Wrana at the nursing home where he lived

  702. I’m on business The report stated that ‘a new funding model is required if the private sector is to be further incentivised, with more funding allocated to private nursing homes in recognition of the specialist services needed to support people with dementia’.

  703. Lost credit card And there are people out there who aren’t using a smartphone or even an iOS or Android phone

  704. In tens, please (ten pound notes) dollar index, a measure of the greenback against abasket of currencies, reached a high of 88.19, its best levelsince June 2010, before slipping back to 87.656, a lossof 0.40 percent on the day.

  705. No, I’m not particularly sporty Senator Graham helped author the Senate’s SB744 mass amnesty scheme, which also doubles legal immigration and puts all enforcement measures in the hands of the corrupt DHS department (which refuses to enforce many existing immigration laws)

  706. Who do you work for? “That certainly confirms the broad, officially stated goal of the X-37B as a test bed vehicle.”

  707. Yes, I play the guitar Then in 2006, Nintendo brought out the Wii, which uses a motion-sensitive remote controller

  708. How do you spell that? The board’s website, though, indicates that a hard copy of the letter itself was sent home, and Prof

  709. I want to make a withdrawal Rolling Stone’s Dana said that the magazine, at Jackie’s request, had not contacted “the man she claimed orchestrated the attack on her nor any of the men she claimed participated in the attack for fear of retaliation against her.”

  710. Special Delivery “The Scottish independence referendum demonstrated people’s enthusiasm for making decisions about the future of the UK

  711. I’ve got a part-time job Radio and television played tributes and downbeat music, newspapers darkened their mastheads, digital billboards were blanked out, and television stations ran tickers announcing his death.

  712. We’re at university together In Diamer, smugglers offer locals 500 to 800 rupees ($5 to $8) per cubic foot of timber, which the smugglers then sells for up to seven times that amount, according to Sher Khan, 30, a member of the village committee in Chilas.

  713. I live here Stethoscopes are unlikely to disappear, but it seems possible that hospital doctors will stop carrying them around

  714. I’m about to run out of credit But this is the book that sums up best his skills as a writer and reporter, his genius as a storyteller

  715. How much were you paid in your last job? I’m a Tamil and there are people dying in my country and you have to like look at it because you’re f—ing Oprah and every American told me you’re going to save the world.’”

  716. How much were you paid in your last job? Sir David has now announced he is to visit the Great Barrier Reef to make a new 3D programme for the BBC, for their first major new commission after they scaled back their 3D projects several years ago.

  717. What are the hours of work? He was there playing good enough to win and he deserve to win and play the final.”

  718. What’s the last date I can post this to to arrive in time for Christmas? But, while the technology is largely there to do this, competition to set a global standard is getting in the way of delivery

  719. Very funny pictures Just being back in the game and the great reception I’ve been getting from the fans, it’s been a pleasant surprise.”

  720. Looking for work Although the potential of a hung Parliament is said to be largely priced in by investors, the reality of what such a situation might mean is arguably not.

  721. An envelope Currently, less than half of people diagnosed with cancer of the mouth or pharynx survive for more than five years

  722. I’d like a phonecard, please The fundamental strategic concern at stake here is about how we secure our future economic prosperity, and with it better jobs and higher living standards for our children and generations to come.

  723. Photography initial public offering (IPO) and by risingconfidence in the economy, companies such online fashionretailer Zalando and start-up firm Rocket Internet arebreathing even more life into equity markets, where volumes areat a seven-year high globally.

  724. What’s the interest rate on this account? That was a sharp drop from the 64,000 added a year ago.Postpaid churn, which is the percentage of those valuablecustomers leaving each month, jumped to 1.31 percent from 1.23percent in the prior quarter.

  725. What do you study? Researchers at the Walter Reed Army Institute of Research in Silver Spring conducted the first human testing of an Ebola vaccine in October

  726. I want to make a withdrawal In an unsigned order, the justices suspended a ruling by a panel of the New Orleans-based U.S

  727. i’m fine good work For example, the adjustable, angled ramp at the heart of the set does more than merely create a series of enigmatic shapes

  728. Will I have to work on Saturdays? She says the tone of the debate in the Commons when two women are at the despatch box is much better than when it's two men.

  729. I want to make a withdrawal Volunteers spent nearly three weeks arranging one million of the coins into a pyramid over one metre tall.

  730. What’s the current interest rate for personal loans? Her high school friends back in Dayton, Ohio sent their donations – some had their heads shaved and some received matching tattoos, to show how much they support the cause for her to stay alive.

  731. Is this a temporary or permanent position? But as well as the message that the spies needed to keep up with modern demands, whether the pace of technological change or for greater accountability from the public, there were also echoes of the past.

  732. Please call back later “Thecompany is trying to tell the market that its management willbecome more professional.”

  733. What do you do? Many have seen similarities because both teams reportedly relied on team and TV revenue to cover debts

  734. I’ll text you later “Vigilantes picked up these weapons and fired back, but they found themselves overwhelmed by the force against them and [they] ran as well,” said Saratu Garba, 20, mother of a two-year-old boy.

  735. Canada>Canada After Reuters asked the bank to look into the account, spokeswoman Jumana Bauwens issued a statement saying: “Bank of America isn’t involved in the transaction.”

  736. I like watching TV Many of the documents salvaged from the 1992 fire are passing through a small room in the National and University Library in Sarajevo, where the Foundation has installed a simple but high-quality rig for digitising the archives.

  737. I was born in Australia but grew up in England In San Francisco, developers are mandated to spend at least 1% of total construction costs on public art but New York has no such rule for private projects.

  738. Have you got any experience? The top-selling item at the auction was a silk overcoat that Monroe often wore in photographs

  739. We used to work together At times the film seems like a job application from veteran Mann (“Collateral,” “Ali,” “The Last of the Mohicans”) and Aussie hunk Hemsworth (aka Thor and the sexiest man alive) to direct and star, respectively, in the next James Bond movie

  740. Could you transfer $1000 from my current account to my deposit account? In protest, the company’s warehouse workers as well as drivers refused to deliver products to the chain’s stores, leading to empty shelves

  741. We work together Economists and real estate agents say a share of 40 percent to 45 percent is required for a strong housing recovery.

  742. I’m in a band The facility will work closely with strategic partners in the Cambridge area such as the Babraham Institute, Wellcome Trust Sanger Institute, the European Bioinformatics Institute, the MRC Laboratory for Molecular Biology and major pharmaceutical and biotechnology companies to bring their different capabilities to bear on clinical research challenges.

  743. Until August It prosecutes suspects accused of genocide, war crimes and other crimes against humanity

  744. I came here to study Senator Sheldon Whitehouse (D-RI) (R) talks to Ron Klain (L), then-Chief of Staff for U.S

  745. I can’t get a dialling tone Our low costs make it possible for seven in 10 full-time undergraduates to attend college tuition-free, fully covered by financial aid and the American Opportunity Tax Credit, and for eight in 10 to graduate free from federal education debt

  746. How much is a First Class stamp? Friends and family recalled a sizable young man — 6-foot-5, nearly 300 pounds — with a gentle, joking manner, a fan of computer games, an aspiring rap musician.

  747. What’s your number? Louis County that the City of Ferguson and former Officer Darren Wilson would be named in the wrongful death lawsuit, which they plan to file promptly.

  748. What part of do you come from? After it was noted that Mississippi was unlikely to change its position on gay marriage in the near future, Higginbotham, an appointee of President Ronald Reagan, appeared to reference that state’s history of racism.

  749. Yes, I love it! I came out of the experience panicky.” In the meantime, he focused on theatre, but came back to film in the early Eighties, starting small and working his way up.

  750. It’s funny goodluck And political analysts largely expect Democrats to lose a handful of House seats.

  751. How much does the job pay? Established organizations — the National Action Network, the NAACP, the National Urban League — have called for people to gather in Washington this Saturday for a national march

  752. Where are you from? Everything in life should have a start point and an end – I hope we're nearing the end of this.”

  753. Could you ask him to call me? Ironically, the fight against Internet regulation gets much of its support from Republicans in sparsely-populated rural states

  754. An estate agents “I think we need a process put in place very quickly that we can point to and rely on as we go forward.”

  755. I study here The committee was meeting just months after the latest Gaza conflict – an inconclusive war that killed more than 2,100 Palestinians, most of them civilians, along with 67 Israeli soldiers and six civilians in Israel.

  756. I came here to study “We always do our due diligence on any player that we are interested in,” Giants co-owner John Mara said in a statement to the Daily News

  757. I’ve just graduated Having won just five golds in 1988, China's programme paid off dramatically, winning 10 times that number in 2008, topping the medals table in its own Beijing Games, and establishing itself as the Olympic powerhouse of the 21st Century.

  758. I’m interested in The sound of dogs barking emanates from inside cars parked near the apartment buildings.

  759. Do you play any instruments? Other conditions are a fact of everyday life here, so the centers stock medicines for those, too: malaria, HIV, parasitic infections, diabetes

  760. Stolen credit card To ease the supply crunch, an additional 13,000 hospitalbeds are needed by 2017, Anahp’s Balestrin said

  761. I’m a member of a gym On the first play next series, Golson hit Fuller 73 yards down the field for a touchdown.

  762. this post is fantastic Holder quickly reassured Republicans when he dropped the Justice Department’s corruption case against Ted Stevens, a former Republican senator from Alaska whom lawmakers had sympathy for

  763. Who would I report to? “Obviously you’re going against some of the best point guards in the world and it’s going to be a lot different going against the backup point guards

  764. An accountancy practice Secretary of State John Kerry said on Friday, but the battle would falter if it becomes consumed by sectarian division or Islamophobia.

  765. Good crew it’s cool 🙂 “Well, you can't hear wheezes with an ultrasound scan, you can't hear chest infections with an ultrasound scan

  766. Can you hear me OK? The only crowded place at Debaltseve on a recent morning was a grocery store where rebels distributed free bread: one loaf per person

  767. I’m not sure Our challenge to companies has resulted in the sector’s biggest ever customer conversation.

  768. Looking for a job He doesn’t want to stop the flights because it then moves the issue squarely to that of closing the Southern border, an issue that has been a Dem/Repub battleground issue for years

  769. I’m interested in King's Fund chief economist Prof John Appleby says while the pledges are welcome “none of them have yet addressed the scale of the financial challenge”.

  770. I’m a housewife We were told by current members of the Conservative Club – where this picture was taken – that it was the local Conservative candidate of the time Edward Jones, who was known as Ed the Ted as he looked like a Teddy Boy.”

  771. I’m on holiday More than half of mothers and one-third of fathers discuss parenting and their child’s health on sites like Facebook or Twitter, a national University of Michigan C.S

  772. What qualifications have you got? In March, Ray Dalio of Bridgewater warned of a re-run of the second leg of the depression in 1937 should the Fed tighten too soon and too hard

  773. Very interesting tale “There were more than 1,000 men from the state's forces, two helicopters and more than 800 paramilitaries,” says Jeihhco, the founder of a cultural centre in Comuna 13

  774. It’s serious “The way my books are structured, everyone was together, then they all went their separate ways and the story deltas out like that

  775. I’m in my first year at university The Tulane research center is about 50 years old, but has been working with such germs since about 2008, CDC officials said.

  776. What qualifications have you got? But as a mother who lost her child, I have no choice but to follow people who are active in our group because they are doing something that I can’t do,” she said.

  777. No, I’m not particularly sporty Nancy Ayres, medical director of non-invasive imaging and director of the fetal cardiology program at Texas Children’s Hospital, first met Rodgers at her 24-week appointment and saw clearly that the left side of Aiden’s heart was completely underdeveloped: Both the left atrium and left ventricle were very small, as well as the mitral valve, which pumps oxygenated blood back out into the body.

  778. I stay at home and look after the children One is an effect where a “false” B-mode signal can be produced on the sky by the CMB passing through massive objects, such as huge galaxies

  779. We used to work together NASA’s heavy-lifting mega-rocket and its ground support systems at Kennedy Space Center in Florida will not be ready for launches until at least mid-2018, even with extra funding approved by Congress, the space agency’s senior human spaceflight official told lawmakers.

  780. I’m doing a masters in law The paper quoted a family friend as saying that Messerschmitt was at work at his firm’s office on Feb

  781. Do you play any instruments? What this amounts to is the Jets paying Harvin $7.1M for the rest of the season in an audition that will determine whether they want to bring him back next year

  782. A few months “The borderline between health and insanity is difficult to see in this kind of case,” he said, adding that diet fads and new lifestyles have made it even more complicated for people to determine what is or is not the right thing to eat.

  783. Why did you come to ? Furthermore, 56% had been affected by a mental health disorder at some point in their lives.

  784. Can you put it on the scales, please? SpaceX, headquartered in Hawthorne, California, also has been contracted by NASA to develop beefed-up Dragons for astronaut rides to the space station, beginning as early as 2017

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